Peer Reviewed: Molecular Beacons: Novel Fluorescent Probes
نویسندگان
چکیده
منابع مشابه
Molecular Beacons and Other Hairpin Probes
Stem-loop (hairpin) DNA oligomer probes provide increased specificity of target recognition as compared with linear DNA probes. Invention of molecular beacons, a variant of stem-loop oligonucleotides, which are able to fluoresce upon hybridization but are ‘‘dark’’ in the absence of the target, made possible real-time monitoring of hybridization process. A variety of robust assays using diverse,...
متن کاملMolecular beacons with intrinsically fluorescent nucleotides
We report the design, synthesis and characterization of a novel molecular beacon (MB-FB) which uses the fluorescent bases (FB) 2-aminopurine (AP) and pyrrolo-dC (P-dC) as fluorophores. Because the quantum yield of these FB depend on hybridization with complementary target, the fluorescent properties of MB-FB were tuned by placing the FB site specifically within the MB such that hybridization wi...
متن کاملReal-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes.
BACKGROUND A number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases. METHODS The introduction of hybridization probes with interactive fluorophore pairs has enabled the development of homogeneous hybridization assays for the direct identifica...
متن کاملMolecular beacons as probes of RNA unfolding under native conditions
Hybridization of fluorescent molecular beacons provides real-time detection of RNA secondary structure with high specificity. We used molecular beacons to measure folding and unfolding rates of the Tetrahymena group I ribozyme under native conditions. A molecular beacon targeted against 15 nt in the 5' strand of the P3 helix specifically hybridized with misfolded forms of the ribozyme, without ...
متن کاملQuadruplex-based molecular beacons as tunable DNA probes.
Molecular beacons (MBs) are fluorescent nucleic acid probes with a hairpin-shaped structure in which the 5' and 3' ends are self-complementary. Due to a change in their emissive properties upon recognition with complementary sequences, MBs allow the diagnosis of single-stranded DNA or RNA with high mismatch discrimination, in vitro and in vivo. Whereas the stems of MB hairpins usually rely on t...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2000
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac003001i